Phase contrast and fluorescence microscopic long time recordings of living cells
Long-time recordings of living cells allow to investigate physiologic processes that occur in the range of minutes to hours. Typical examples are cell migration, differentiation and growth. In combination with multicolor fluorescent recordings, it additionally allows to investigate the changes in the distribution of cellular proteins.
Example recordings
Live cell recording of migrating and proliferating oligodendrocyte progenitor cells. Recorded at 2 frames per minute, played at 24 fps, total number of frames: 564.
Mutli-channel fluorescent recording of a neural culture from rat brain. Green: Staining against myelin/oligodendrocyte-specific protein (MOSP, showing mature oligodendrocytes), red: glial fibrillary acidic protein (GFAP, showing astrocytes) and blue: DNA (showing all cells).