Ouabain binding study on cell cultures from the central nervous system
In the course of this project cell cultures will be obtained from postnatal rats. These cultures primarily composed of neurons and astrocytes will be treated with growth factors or other proteins and factors that are capable of modulating neuronal excitability. Our aim is to test the hypothesis that an increased excitability in neurons also leads to an upregulated expression of Na+/K+-ATPase. We use Tritium-labeled ouabain which selectively binds to the Na+/K+-ATPase to determine the density of this enzyme in the cell membrane.