Transport and insertion mechanisms of plastid-encoded thylakoid membrane proteins

The biogenesis of photosynthetic protein complexes of the chloroplast thylakoid membrane requires highly specific protein sorting, integration and assembly mechanisms of nucleus as well as plastid encoded subunits. Central steps in the biogenesis of photosystem II (PS II) are the cotranslational insertion of the plastid encoded D1 protein into the thylakoid membrane and its subsequent assembly into functional PS II. We recently established a technique to partially reconstitute the cotranslational insertion of [35S]-D1 using a homologous in vitro translation system derived from pea chloroplasts. The aims of this proposal are (I) to identify novel components involved in cotranslational protein insertion in thylakoid membranes, (II) to dissect the protein contacts of the nascent D1 chain during translation and insertion and (III) to get insight into the mechanisms underlying targeting and attachment of ribosome-nascent chain complexes to the thylakoid membrane.

Funded by

  • Deutsche Forschungsgemeinschaft (SCHU 1163/6-2 (FOR2092)): Transport and insertion mechanisms of plastid-encoded thylakoid membrane proteins

Publications

  • Hristou A et al.: Ribosome-associated chloroplast SRP54 enables efficient co-translational membrane insertion of key photosynthetic proteins. Plant Cell 2019 DOI: 10.1105/tpc.19.00169

Theses

This project has no theses.