Newsportal - Ruhr-Universität Bochum
Research
Projects
We are currently working on these projects:
- Development of STED and SICM instruments
- Development of a combined stimulated emission depletion and scanning ion conductance microscope
- Investigating the growth-cone-like structures at the tips of oligodendrocyte progenitor cell processes
- Ouabain binding study on cell cultures from the central nervous system
- Evaluating Triton-X-100 Alternatives in Immunocytochemical Staining Protocols
- Patch Clamp Recordings of Neural Cells
- Expansion Microscopy
- Calcium Imaging of Neural Cells
Publications
- Annika Haak* , Heiko M. Lesslich, Irmgard D. Dietzel (2023) Visualization of the membrane surface and cytoskeleton of oligodendrocyte progenitor cell growth cones using a combination of scanning ion conductance and four times expansion microscopy. Biological Chemistry 405(1). doi: 10.1515/hsz-2023-0217
- Happel P, Haak A*, Gesper A (2022) Correlating Scanning Ion Conductance and Super-Resolved Fluorescence Microscopy. Bioanalytical Reviews 3. doi: 10.1007/11663_2022_13
- Lesslich, HM, Klapal, L, Wilke, J, Haak, A, Dietzel, ID (2022) Adjusting the neuron to astrocyte ratio with cytostatics in hippocampal cell cultures from postnatal rats: A comparison of cytarabino furanoside (AraC) and 5-fluoro-2’-deoxyuridine (FUdR). PLOS ONE 17. doi: 10.1371/journal.pone.0265084
- Mohana Sundaram, S, Marx, R, Lesslich, HM, Dietzel, ID (2022) Deficiency of Thyroid Hormone Reduces Voltage-Gated Na+ Currents as Well as Expression of Na+/K+-ATPase in the Mouse Hippocampus. International Journal of Molecular Sciences 23. doi: 10.3390/ijms23084133
- Gesper, A, Wennmalm, S, Hagemann, P, Eriksson, SG, Happel P, Parmryd, I (2020) Variations in Plasma Membrane Topography Can Explain Heterogenous Diffusion Coefficients Obtained by Fluorescence Correlation Spectroscopy. Frontiers in Cell and Developmental Biology 8. doi: 10.3389/fcell.2020.00767
- Hagemann, P, Gesper, A, Happel, P (2018) Correlative Stimulated Emission Depletion and Scanning Ion Conductance Microscopy. ACS Nano 12. doi: 10.1021/acsnano.8b01731
- Gesper, A, Hagemann, P, Happel, P (2017) A low-cost, large field-of-view scanning ion conductance microscope for studying nanoparticle–cell membrane interactions. Nanoscale 9. doi: 10.1039/c7nr04306f
- Thatenhorst, D, Rheinlaender, J, Schäffer, TE, Dietzel, ID, Happel P (2014) Effect of Sample Slope on Image Formation in Scanning Ion Conductance Microscopy. Analytical Chemistry 84. doi: 10.1021/ac5024414
- Happel, P, Hoffmann G, Mann SA, Dietzel ID (2003) Monitoring cell movements and volume changes with pulse-mode scanning ion conductance microscopy. Journal of Microscopy 212. doi: 10.1046/j.1365-2818.2003.01248.x